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1x mem non-essential amino acids solution (neaa)  (Thermo Fisher)


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    Thermo Fisher 1x mem non-essential amino acids solution (neaa)
    1x Mem Non Essential Amino Acids Solution (Neaa), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mem+non-essential+amino+acids+solution+(neaa)/1%C3%97+mem+non+essential+amino+acids/pm40550888-195-22-35
    Average 90 stars, based on 1 article reviews
    1x mem non-essential amino acids solution (neaa) - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Pump‐Less, Recirculating Organ‐on‐Chip (rOoC) Platform to Model the Metabolic Crosstalk between Islets and Liver
    Article Snippet: For further maturation, organoids were cultured in microwells in William's E media, supplemented with 1% (v/v) ITS, 0.1 μM dexamethasone, 20 ng mL −1 Oncostatin M, and 1% (v/v) MEM non‐essential amino acids solution (NEAA, Thermo Fisher Scientific, catalog no. 11140050), 10 μM DAPT for another 10 days with the replacement of media every 48 h.

    Article Title: Telomerase Reverse Transcriptase Promoter Mutations in Human Hepatobiliary, Pancreatic and Gastrointestinal Cancer Cell Lines.
    Article Snippet: Hep3B and HepG2 cells were grown in MEM supplemented with 10% FBS and 1% MEM non-essential amino acids solution (NEAA; Thermo Fisher Scientific).



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    GLS1-mediated glutamine metabolism maintains cell proliferation by providing proliferative response substrates. ( A ) Representative bright-field images of liver organoids before (upper panel) and after (lowing panel) the treatment with or without CB839 and <t>NEAA</t> for 48 h (Bar = 1000 μm) and the quantification of organoid size ( B ). ( C ) Cell viability measurement was performed using presto blue. Relative cell viability was calculated by normalizing to Control after 48 h of incubation ( n = 5). ( D ) Protein synthesis in liver organoids treated with or without CB839 and NEAA for 48 h was determined by L-homopropargylglycine (HPG) mean fluorescence intensity (MFI), ( n = 5). (E) Proliferation of liver organoids treated with or without CB839 and NEAA for 3 h was determined by BrdU incorporation, ( n = 5). All data are means ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, one-way ANOVA was used
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    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy <t>medium</t> (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: <t>100</t> μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
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    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy <t>medium</t> (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: <t>100</t> μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
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    Image Search Results


    GLS1-mediated glutamine metabolism maintains cell proliferation by providing proliferative response substrates. ( A ) Representative bright-field images of liver organoids before (upper panel) and after (lowing panel) the treatment with or without CB839 and NEAA for 48 h (Bar = 1000 μm) and the quantification of organoid size ( B ). ( C ) Cell viability measurement was performed using presto blue. Relative cell viability was calculated by normalizing to Control after 48 h of incubation ( n = 5). ( D ) Protein synthesis in liver organoids treated with or without CB839 and NEAA for 48 h was determined by L-homopropargylglycine (HPG) mean fluorescence intensity (MFI), ( n = 5). (E) Proliferation of liver organoids treated with or without CB839 and NEAA for 3 h was determined by BrdU incorporation, ( n = 5). All data are means ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, one-way ANOVA was used

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis

    doi: 10.1007/s00018-025-05772-z

    Figure Lengend Snippet: GLS1-mediated glutamine metabolism maintains cell proliferation by providing proliferative response substrates. ( A ) Representative bright-field images of liver organoids before (upper panel) and after (lowing panel) the treatment with or without CB839 and NEAA for 48 h (Bar = 1000 μm) and the quantification of organoid size ( B ). ( C ) Cell viability measurement was performed using presto blue. Relative cell viability was calculated by normalizing to Control after 48 h of incubation ( n = 5). ( D ) Protein synthesis in liver organoids treated with or without CB839 and NEAA for 48 h was determined by L-homopropargylglycine (HPG) mean fluorescence intensity (MFI), ( n = 5). (E) Proliferation of liver organoids treated with or without CB839 and NEAA for 3 h was determined by BrdU incorporation, ( n = 5). All data are means ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, one-way ANOVA was used

    Article Snippet: Rescue experiments were performed with 5 mM glutathione reduced ethyl ester (GSH-EE), 1x MEM Non-Essential Amino Acids solution (NEAA) from Thermo Fisher Scientific.

    Techniques: Control, Incubation, Fluorescence, BrdU Incorporation Assay

    Schematic model illustrating the effect of the swift of GLS2 to GLS1 on ROS-Wnt/β-catenin signaling and proliferation in liver organoids. GLS1 reduces the generation of ROS, promotes nuclear translocation of β-Catenin and then promotes Wnt/β-Catenin target genes transcription in liver organoids. In addition, GLS1 accelerates the metabolism of Glutamine and provides proliferative response substrates such as amino acids and nucleotides for cell proliferation. Abbreviations: Gln, Glutamine; GSH, Glutathione; Glu, Glutamate; ROS, Reactive oxygen species; NEAA: non-essential amino acids

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis

    doi: 10.1007/s00018-025-05772-z

    Figure Lengend Snippet: Schematic model illustrating the effect of the swift of GLS2 to GLS1 on ROS-Wnt/β-catenin signaling and proliferation in liver organoids. GLS1 reduces the generation of ROS, promotes nuclear translocation of β-Catenin and then promotes Wnt/β-Catenin target genes transcription in liver organoids. In addition, GLS1 accelerates the metabolism of Glutamine and provides proliferative response substrates such as amino acids and nucleotides for cell proliferation. Abbreviations: Gln, Glutamine; GSH, Glutathione; Glu, Glutamate; ROS, Reactive oxygen species; NEAA: non-essential amino acids

    Article Snippet: Rescue experiments were performed with 5 mM glutathione reduced ethyl ester (GSH-EE), 1x MEM Non-Essential Amino Acids solution (NEAA) from Thermo Fisher Scientific.

    Techniques: Translocation Assay

    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy medium (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: 100 μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.

    Journal: Bio-protocol

    Article Title: A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)

    doi: 10.21769/BioProtoc.5304

    Figure Lengend Snippet: (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy medium (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: 100 μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.

    Article Snippet: MEM non-essential amino acids solution (NEAA) (100×) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 12.

    Techniques: Derivative Assay, Control, Staining, Marker, Expressing